Journal: Biomolecules

Article Title: Pterostilbene Enhances Cytotoxicity and Chemosensitivity in Human Pancreatic Cancer Cells

doi: 10.3390/biom10050709

Figure Lengend Snippet: Effect of pterostilbene on autophagy-related protein expression in MIA PaCa-2 cells and MIA PaCa-2 GEMR cells. Cells were treated with 50 and 75 μM pterostilbene for 72 h, and the expression levels of Atg5, Beclin-1 and LC3 II protein in ( A ) MIA PaCa-2 and ( E ) MIA PaCa-2 GEMR cells were analyzed by western blotting. The relative expression levels of ( B , F ) Atg5, ( C , G ) Beclin-1, and ( D , H ) LC3 II are expressed as the mean ± SD ( n = 3). p values were considered statistically significant when * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the untreated control.

Article Snippet: Microtubule-associated protein light chain 3 II (LC3 II) antibody, peroxidase goat anti-mouse and peroxidase goat anti-rabbit were purchased from GeneTex (Irving, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: Sample size calculation.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques:

Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: ( A – D ) Protein analysis by Western blotting. ( A ) LC3 I and II (Molecular weight: 18 kDa and 16 kDa, respectively). ( B ) p62 (Molecular weight: 62 kDa). ( C ) Caspase-3: 32 kDa). ( D ) β-actin: 42 kDa). ( E – H ): LC3II, LC3II/LC3I ratio, P62 and caspase3 proteins relative quantitation /β-actin ratio) by Western blotting. Data are mentioned as mean ± SE; different letters = significant difference). LC3: Microtubule-associated proteins 1A/1B light chain 3B. M: Marker. MW: molecular weight.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques: Western Blot, Molecular Weight, Quantitation Assay, Marker

Journal: Cells

Article Title: Enhanced Autophagic Flux, Suppressed Apoptosis and Reduced Macrophage Infiltration by Dasatinib in Kidneys of Obese Mice

doi: 10.3390/cells11040746

Figure Lengend Snippet: ( A – D ): Immunohistochemical staining for LC3 (×400) in the kidney tissues of vehicle, Dasatinib control, Obesogenic diet and Obesogenic diet + Dasatinib groups ( A – D , respectively). Scale bar = 50 µm. ( E ) The mean percentage of LC3 immunopositive area. Results are presented as mean ± standard error. Different letters mean significant difference. Arrows: LC3 + ve cells. LC3 = Microtubule-associated protein light chain 3.

Article Snippet: After blocking, an overnight membrane incubation with the primary antibodies—rabbit polyclonal antibody for LC3 and rabbit polyclonal antibody for SQSTM1/P62 as markers for autophagic activity, mouse monoclonal antibody for caspase-3 as an apoptosis marker and β-actin as a control protein (Biospes; YPA1652, ABclonal; A11250, Santa Cruz sc-7272 and Abcam ab227387, respectively)—was performed overnight at 4 °C (1:500, 1:1000, 1:100 and 1:5000 dilutions, respectively).

Techniques: Immunohistochemical staining, Staining, Control

Journal: Oncology Reports

Article Title: β2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy

doi: 10.3892/or.2019.7266

Figure Lengend Snippet: Correlation of β1-AR with autophagic markers.

Article Snippet: LC3-II (cat. no. A11923) for IHC staining was purchased from Abclonal.

Techniques:

Journal: Oncology Reports

Article Title: β2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy

doi: 10.3892/or.2019.7266

Figure Lengend Snippet: Correlation of β2-AR with autophagic markers.

Article Snippet: LC3-II (cat. no. A11923) for IHC staining was purchased from Abclonal.

Techniques: